生长分化因子9在牛卵丘卵母细胞复合体体外成熟过程中的表达

doi:10.11843/j.issn.0366-6964.2013.10.009

Abstract

Abstract:

The relationship between GDF-9 gene expression and expansion of COCs during in vitro maturation was investigated in this study. The expression of GDF-9 in bovine COCs during in vitro maturation(IVM)( 0, 6, 12, 18, 24 and 28 h) were detected by fluorescent quantitative RT-PCR method. The cumulus expansion was also observed. The RT-PCR result showed that GDF-9 mRNA expressed regularly in bovine COCs during in vitro maturation, which increased from 0-12 h culture and attained the highest expression at 12 h maturation culture, but subsequently decreased gradually and attained the lowest expression at 24 h maturation culture. The cumulus cells of COCs began to expand apparently at 12 h IVM culture, and increased expansion with maturation culture afterwards.The rusults showed that cumulus cells begin to expansion when expression of GDF-9 mRNA was highest in COCs, which suggested that GDF-9 may play essential role in cumulus expansion and oocyte maturation of bovine.

CLC Number:

S823
S814.8

OU YANG Xiao-qing, YANG Shu-qing, ZHANG Chun-qiang, LI Rui-lan, ZHANG Tong, ZHANG Jia-xin. Expression of Growth Differentiation Factor-9 in Bovine COCs during in vitro Maturation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1561-1568.

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Expression of Growth Differentiation Factor-9 in Bovine COCs during in vitro Maturation

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